Table 1 List of the constructed Lip2 surface display systems and the generated novel recombinant Y. lipolytica strains in this study
pYL constructs | Promoter | Int. site | Gene |
|---|---|---|---|
Native promoters with one gene copy | |||
EXP1-YlPIR1-mCherry IntE_4 | EXP1 | IntE_4 | mCherry |
EXP1-YlPIR1-mCherry IntF_3 | EXP1 | IntF_3 | mCherry |
H3-YlPIR1-mCherry IntF_3 | H3 | IntF_3 | mCherry |
EXP1-YlPIR1-LIP2 IntE_4 | EXP1 | IntE_4 | LIP2 |
EXP1-YlPIR1-LIP2 IntF_3 | EXP1 | IntF_3 | LIP2 |
H3-YlPIR1-LIP2 IntF_3 | H3 | IntF_3 | LIP2 |
Native promoters with two gene copies | |||
EXP1-YlPIR1-mCherry IntE_4/EXP1-YlPIR1-mCherry IntF_3 | EXP1 EXP1 | IntE_4 IntF_3 | mCherry mCherry |
EXP1-YlPIR1-mCherry IntE_4/H3-YlPIR1-mCherry IntF_3 | EXP1 H3 | IntE_4 IntF_3 | mCherry mCherry |
EXP1-YlPIR1-mCherry IntE_4/H3-EGFP IntF_3 | EXP1 H3 | IntE_4 IntF_3 | mCherry EGFP |
EXP1-YlPIR1-LIP2 IntE_4/EXP1-YlPIR1-LIP2 IntF_3 | EXP1 EXP1 | IntE_4 IntF_3 | LIP2 LIP2 |
EXP1-YlPIR1-LIP2 IntE_4/H3-YlPIR1-LIP2 IntF_3 | EXP1 H3 | IntE_4 IntF_3 | LIP2 LIP2 |
Hybrid promoters with one gene copy | |||
UAS1B8-TMAL(250)-YlPIR1-mCherry IntC_2 | UAS1B8-TMAL(250) | IntC_2 | mCherry |
UAS1B8-TMAL(250)-YlPIR1-LIP2 IntC_2 | UAS1B8-TMAL(250) | IntC_2 | LIP2 |