Fig. 8
Effect of carbon source on the enzymatic activity of the UAS1B8-TMAL(250)-YlPIR1-LIP2 IntC_2 biomass. Biomass was harvested after 72 h of cultivation in flasks (30 °C, 150 rpm) and lyophilized. One unit of biomass activity was defined as 1 μmol of p-nitrophenol released per minute at 40 °C using 10-mM pNPP and 1-Μ butanol in hexane as substrates. Data points represent the mean values of two biological replicates (n = 2), with standard deviations shown as error bars