Fig. 6

Monitoring of OD600nm, glycerol concentration, Lip2 activity, dissolved oxygen (pO2), and pH in bioreactor fermentations of recombinant Y. lipolytica UAS1B8-TMAL(250)-YlPIR1-LIP2 IntC_2. The pO2 control system enabled automatic adjustment of agitation under two conditions: A High-speed agitation (500–800 rpm) and B low-speed agitation (150–300 rpm). Data points represent the mean values of three biological replicates (n = 3), with standard deviations shown as error bars