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Fig. 5 | Biotechnology for the Environment

Fig. 5

From: Engineering novel Yarrowia lipolytica whole-cell biocatalysts by cell surface display of the native Lip2 lipase for biodiesel production

Fig. 5

Screening of recombinant Y. lipolytica strains on tributyrin agar plates. A Cultures of recombinant Y. lipolytica strains grown on YPG agar at 24, 48, and 72 h. B Supernatants of recombinant Y. lipolytica cultures after 72-h incubation at 30 °C and 150 rpm. Strains are arranged in a clockwise direction: (center) WT MUCL 28849 as control; 1) EXP1-YlPIR1-LIP2 IntE_4. 2) H3-YlPIR1-LIP2 IntF_3. 3) EXP1-YlPIR1-LIP2 IntF_3. 4) EXP1-YlPIR1-LIP2 IntE_4/EXP1-YlPIR1-LIP2 IntF_3. 5) EXP1-YlPIR1-LIP2 IntE_4/H3-YlPIR1-LIP2 IntF_3. 6) UAS1B8-TMAL(250)-YlPIR1-LIP2 IntC_2

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Biotechnology for the Environment

ISSN: 2948-2356

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