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Fig. 3 | Biotechnology for the Environment

Fig. 3

From: Engineering novel Yarrowia lipolytica whole-cell biocatalysts by cell surface display of the native Lip2 lipase for biodiesel production

Fig. 3

Fluorescence images obtained using confocal laser scanning microscopy (CLSM). CLSM allowed the simultaneous detection of fluorescent cells and the localization of the membrane mCherry and the cytoplasmic EGFP protein of A wild-type Y. lipolytica MUCL 28849 as control, B EXP1-YlPIR1-mCherry IntE_4, C EXP1-YlPIR1-mCherry IntF_3, D H3-YlPIR1-mCherry IntF_3, E UAS1B8-TMAL(250)-YlPIR1-mCherry IntC_2, F EXP1-YlPIR1-LIP2 IntE_4/EXP1-YlPIR1-LIP2 IntF_3, G EXP1-YlPIR1-LIP2 IntE_4/H3-YlPIR1-LIP2 IntF_3, and H EXP1-YlPIR1-mCherry IntE_4/H3-EGFP IntF_3 after 72-h cultivation in YPG at 30 °C and 150 rpm

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Biotechnology for the Environment

ISSN: 2948-2356

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