Fig. 1

Maps of the integrative expression cassettes for the Lip2 surface display systems for Y. lipolytica constructed in this study. A General map of the integrative pYL vector constructed for the expression of genes of interest on cell surface. B Map of integrative pYLH3-EGFP IntF_3 for the expression of EGFP gene and the localization of the protein in the cytoplasm of yeast cells. Expression cassettes were confirmed by double restriction enzyme digestion and Sanger sequencing