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Table 2 Overview of the treatment procedures applied to M. brunneum blastospores in the microfluidic chip system

From: Solute excretion improves trehalose uptake and desiccation tolerance of Metarhizium brunneum blastospores

Media changes

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Untreated

Trehalose treatment

Hypotonic + trehalose treatment

0

7 M MgCl2 (∞)

Medium (∞)

Medium (2 h)

Medium (2 h)

Medium(2 h)

1

-

-

7 M MgCl2 (2 h)

100 g/L trehalose(20 min)

12,32 mM NaCl (20 min)

2

-

-

Medium (∞)

7 M MgCl2 (2 h)

100 g/L trehalose (20 min)

3

-

-

-

Medium (∞)

7 M MgCl2 (2 h)

4

-

-

-

-

Medium (∞)